Postzygotic Isolation Evolves before Prezygotic Isolation between Fresh and Saltwater Populations of the Rainwater Killifish, Lucania parva

Table 1

Analyses of prezygotic isolation between L. parva populations. Results of general linear model for (a) latency to mate, (b) total number of eggs produced. Male population (Pecos, Indian River), female population (Pecos, Indian River), and their interaction are included as factors. Prezygotic isolation predicts an interaction between male population and female population. Statistically significant values are indicated in bold.

(a) Latency to mate

	Outlier included			Outlier removed
Source	df	F	P	df	F	P
Male population	1,28	0.32	0.58	1,27	0.34	0.56
Female population	1,28	0.52	0.48	1,27	9.31	0.0051
Male * Female population	1,28	1.17	0.29	1,27	0.18	0.68

(b) Total number of eggs produced

Source	df	F	P
Male population	1,28	0.08	0.78
Female population	1,28	<0.01	0.96
Male * Female population	1,28	2.57	0.12