Alternative Splicing: A Potential Source of Functional Innovation in the Eukaryotic Genome

Figure 3

Percentage of AS areas containing identifiable functional domains, secondary structures, and stop codons in human. Functional components were identified using InterProScan which contains 14 applications for the prediction of protein domains [17], including Pfam for the prediction of protein domains [18], SignalP 3.0 for signal peptide predictions [19], and TMHMM [20] for the predictions of transmembrane domains. PSORT II [21] was used to identify the likely subcellular localization of protein products. Secondary protein structures were predicted by CLC Main Workbench 5.7, which is based on extracted protein sequences from the protein databank (http://www.rcsb.org/pdb/).