Repeated Evolution of Testis-Specific New Genes: The Case of Telomere-Capping Genes in Drosophila
Table 1
hiphop and K81-like genes in Drosophila.
Species\gene
ID# or GI#
Orthologous region in D. mel
PTV/QFVH motif
Position of putative start codon
Position of stop codon
D. mel\hiphop
CG6874
D.mel hiphop
RRPTV -LDKQSMD
D. mel\K81
CG14251
D.mel K81
RRQFVH LNREAMA
D. sim\hiphop
GD14769
D.mel hiphop
RRPTV -LDKPSMD
D. sim\K81
GD21311
D.mel K81
RRQFVH LNHQAMA
D. bia\hiphop
358392949
D.mel hiphop
RRPTVH LNKEAMD
690387
689698
D. bia\K81
358402098
D.mel K81
RRQFIH LNKEAMD
2964671
2965297
D. tak\K81
343975433
D.mel K81
RRQFVH LNKEAMD
141804
141217
D. tak\hiphop-like
343974900
chro2L in fred gene
RRQFVH LNKEAMD
211517
212122
D. rho\hiphop
358405427
D.mel hiphop
RRYVP -LNKVAMD
33547
32867
D. rho\K81-like
358404732
chroX in Sh gene
RRQFVH LNKEAMD
683852
683265
D. rho\hiphop-K81-like-1
358405183
chroX Roc1a/CG13367
RRFVA -PNKEVMD
799350
800057
D. ele\hiphop
343972741
D.mel hiphop
RRQVVH PNKKAMD
ND
1725959
D. ele\K81-like
343972552
chroX in Sh gene
RRQFVH LNKNAMD
34447
35022
D. ele\hiphop-K81-like-1
343972719
chroX Roc1a/CG13367
RRPTTI LNKESMD
1005656
1006243
D. eug\hiphop
358409234
D.mel hiphop
RRPVTH LNKEAMD
677060
676191
D. eug\K81
358409002
D.mel K81
RRQFVH LNKEAME
154852
155409
D. fic\K81
343464569
D.mel K81
RRSFVH LNKEAMD
2599414
2600109
D. fic\hiphop-K81-like-1
343464682
chroX Roc1a/CG13367
RRPTVH LNKEAMD
461020
461505
D. fic\hiphop-K81-like-2
343464675
chro2L CG34163/zuc
RRPALH LNKEAMD
185420
184971
D. kik\hiphop-like
343973849
chro2L bsf/CR43344
RRQFLV PNKKVMD
92534
92040
D. ana\hiphop
GF10272
chro3L YT521-B/Drs
RRPTII LNKAVMD
D. bip\hiphop
358403122
chro3L YT521-B/Drs
RRQTVI LNKAAMD
1284107
1283427
D. pse\hiphop
GA19922
D.mel hiphop
RRQVVH LNKTAMD
D. wil\hiphop
GK12110
D.mel hiphop
RRQIQ -LTGPHLD
D. wil\hiphop-like
GK15167
chro2L Or33c/Cry
RRQVN -RSGIDLD
D. moj\hiphop-like
GI17239
chro2L CG13398
RRQFVH LNKDVMD
D. vir\hiphop-like
GJ17998
chro2L CG13398
RRQFVH LNKDVMD
D. gri\hiphop-like
GH13489
chro2L CG13398
RRQFVS LNKDVMD
The hiphop and K81-like genes were identified by tBLASTn search in Flybase (http://flybase.org/blast/). For each gene, the ID number, when available, is indicated. For species whose genome is not yet annotated, a GI number corresponding to the scaffold DNA sequence is indicated with the position in the scaffold of the putative start codon (first methionine in phase with the homolog protein identified) and the stop codon. ND: not determined. The orthologous region in the D. melanogaster genome surrounding the hiphop-K81-like gene is indicated as follows: chromosome and neighbor genes. When two genes are indicated, the hiphop-K81-like gene is placed in between. D. mel hiphop and D. mel K81 means that the synteny block is conserved between the gene of interest and hiphop or K81 from D.  melanogaster, respectively. The PTV or QFVH motifs of the HipHop/K81 proteins as defined by Gao et al. [58] are highlighted in bold. D. mel: Drosophila melanogaster; D. sim: D. simulans; D. bia: D. biarmipes; D. tak: D. takahashi; D. rho: D. rhopaloa; D. ele: D. elegans; D. eug: D. eugracilis; D. fic: D. ficusphila; D. kik: D. kikkawai; D. ana: D. ananassae; D. bip: D. bipectinata; D. pse: D. pseudoobscura; D. wil: D. willistoni; D. moj: D. mojavensis; D. vir: D. virilis; D. gri: D. grimshawi.
