Evolution of Three Parent Genes and Their Retrogene Copies in Drosophila Species

Figure 3

Expression patterns of CG8331 and CG4960. Expression of CG8331 and CG4960 was detected in Drosophila species by in situ hybridization as shown in the top and bottom panels, respectively. Purple coloration indicates presence of mRNA. Cladograms show the relationships of the species investigated. Numbers on individual panels indicate the number of embryos assessed with consistent staining patterns. White arrows point to salivary glands. Stage ranges are according to [24]: 0–2, syncytial blastoderm; 3-4, cellular blastoderm; 9-10, during germ-band elongation; 14–16, final embryonic stages. Staining during stages 0–2 indicates presence of maternal transcripts [25]. Dmel, D. melanogaster; Dsim, D. simulans; Dyak, D. yakuba; Dana, D. ananassae; Dpse, D. pseudoobscura; Dvir, D. virilis.