Table of Contents
International Journal of Evolutionary Biology
Volume 2013, Article ID 693085, 12 pages
Research Article

Evolution of Three Parent Genes and Their Retrogene Copies in Drosophila Species

Department of Biology, University of New Brunswick, 10 Bailey Drive, Fredericton, NB, Canada E3B 5A3

Received 28 February 2013; Accepted 8 May 2013

Academic Editor: Shozo Yokoyama

Copyright © 2013 Ryan S. O'Neill and Denise V. Clark. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary File 1 contains a list of alternative transcripts for CG8331, CG4960, CG17734, CG11825, Sep2, and Sep5 in Drosophila melanogaster; pairwise sequence alignments; presence or absence of each gene within the 20 Drosophila genome sequences; a description of all orthologs of the six genes within the 20 Drosophila species; primer sequences used to detect transcription using qRT-PCR; primer sequences used to make probes for in situ hybridization; data from the modENCODE developmental transcriptome of D. melanogaster ; expression data from FlyAtlas; PAML FEL, and MEME results.

Supplementary Figures 2, 3, and 4 contain annotated multiple sequence alignments for the three gene pairs.

Supplementary Figure 5 contains graphs of the expression levels of the three gene pairs as detected by qRT-PCR.

Supplementary File 6 contains the multiple sequence alignments of the three gene pairs in Clustal format.

Supplementary Figure 7 contains examples of background staining for each probe, as determined by using sense probes for in situ hybridization.

  1. Supplementary Materials