Table of Contents
International Journal of Evolutionary Biology
Volume 2015, Article ID 538918, 11 pages
Research Article

The Evolutionary History of Daphniid α-Carbonic Anhydrase within Animalia

1Program in Ecology & Evolutionary Biology, Department of Biology, University of Oklahoma, 730 Van Vleet Oval, Norman, OK 73019, USA
2University of Oklahoma Biological Station, 15389 Station Road, Kingston, OK 73439, USA
3Murray State College, One Murray Campus, Tishomingo, OK 73460, USA

Received 5 October 2014; Revised 12 March 2015; Accepted 14 March 2015

Academic Editor: Hirohisa Kishino

Copyright © 2015 Billy W. Culver and Philip K. Morton. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary material that follows includes the following tables and figures: Tables S1, S2, S3, and Figure S1.

We constructed Table S1 to list the taxa with their respective isoforms of α-Carbonic Anhydrase (CA) that were used in both the MrBayes and RaxML phylogenetic analysis. Since, branches of the resultant phylogenies are collapsed for clarification of the figures, it was necessary to provide a list of the taxa used. We also provide the NCBI ascension number of the CAs used.

Table S2 is listing of the conserved amino acid residues among the α-CA isoforms and taxonomical groupings. It was compiled using the alignment of the amino acid sequences (see Material and Methods). Residues that are conserved within α-CA groupings are highlighted by a color based upon which grouping they are associated (see Table legend).

Table S3 list the ancestral states of informative amino acid residues (see Materials and Methods for criteria) based upon the phylogeny. Each node of the phylogeny is represented in the table with the predicted ancestral amino acid sequence. The ancestral states were inferred using a maximum likelihood approach.

Figure S1 is the result of our RaxML phylogenetic analysis. This tree has a similar topology as the MrBayes tree that is printed in the main manuscript. This tree was run to verify or MrBayes results. The main difference between these two trees is that the RaxML tree resolves the polytomy in the vertebrate cytosolic α-CAs, but with low support.

  1. Supplementary Materials