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International Journal of Electrochemistry
Volume 2011 (2011), Article ID 470607, 7 pages
http://dx.doi.org/10.4061/2011/470607
Research Article

A Biocompatible Nanocomposite for Glucose Sensing

1Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, Tehran 1417614411, Iran
2Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran 1417614411, Iran

Received 7 February 2011; Accepted 28 February 2011

Academic Editor: Sibel A. Ozkan

Copyright © 2011 Parvaneh Rahimi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A nanocomposite containing amine functionalized multiwalled carbon nanotubes and a room temperature ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate) was prepared and applied for glucose oxidase (GOx) immobilization on glassy carbon electrode. The proposed nanocomposite provided a favorable microenvironment to preserve the bioactivity of GOx. It could also effectively facilitate the enzyme direct electron transfer to the electrode. This brought about a remarkable improvement in the sensitivity of the glucose biosensor. Under the optimum experimental conditions, the formal potential of GOx was about −467.5 mV. Moreover, the sensitivity and response time of the biosensor toward glucose were 1277 μA mM-1 cm-2 and 6 s, respectively. The biosensor provided a linear dynamic response between 0.1 and 43 μM with a very low detection limit of 63 nM. Also, the values for apparent Michaelis-Menten constant and maximum current were obtained as 18 μM and 2.7 μA, respectively.