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International Journal of Electrochemistry
Volume 2011 (2011), Article ID 603257, 4 pages
Research Article

Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor

1University of Carthage, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, BP 676, 1080 Charguia Cedex, Tunisia
2Unité de Recherche de Physico-Chimie des Matériaux Polymères, IPEST, Université de Carthage, La Marsa 2070, Tunisia

Received 30 March 2011; Accepted 30 April 2011

Academic Editor: Farnoush Faridbod

Copyright © 2011 H. Baccar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The amino groups can be activated with glutaraldehyde for horseradish peroxidase immobilization. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio attained with palladium nanoparticles. The second biosensor presents a better detection limit of 7.5 μM in comparison with the first one which is equal to 75 μM.