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International Journal of Electrochemistry
Volume 2012 (2012), Article ID 183745, 10 pages
http://dx.doi.org/10.1155/2012/183745
Research Article

Interaction of Calf Thymus DNA with the Ni(II) Complex of Sodium 1,4-Dihydroxy-9,10-Anthraquinone-2-Sulphonate: A Novel Method of Analysis Using Cyclic Voltammetry

1Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF-Bidhannagar, Kolkata 700064, India
2Department of Chemistry, Jadavpur University, Raja S. C. Mullick Road, Kolkata 700032, India
3Department of Chemistry, Shibpur Dinobundhoo Institution (College), 412/1 G. T. Road (South), Howrah 711102, India

Received 15 February 2011; Accepted 20 April 2011

Academic Editor: Rene Kizek

Copyright © 2012 Partha Sarathi Guin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hydroxy-9,10-anthraquinones are cheaper alternatives to anthracycline drugs. They closely resemble anthracycline drugs both from a structural and functional viewpoint. Electrochemical behavior of the Ni(II) complex (Na2[Ni(NaLH)2Cl2]⋅2H2O) of sodium 1,4-dihydroxy-9,10-anthraquinone-2-sulphonate (NaLH2), analogue of the core unit of anthracycline anticancer drugs, was studied at physiological pH using cyclic voltammetry. The Ni(II) complex of sodium 1,4-dihydroxy-9,10-anthraquinone-2-sulphonate undergoes diffusion-controlled one-electron reduction that enables performing an electrochemical study on the interaction of the complex with calf thymus DNA. The complex was titrated with increasing concentrations of DNA, and the reduction peak for the unbound complex helped in evaluating binding parameters. Analysis of binding data using nonlinear curve fit in a cyclic voltammetry experiment is the first such attempt. The paper evaluates site size of interaction that also serves as a means to determine stoichiometry of complex formation, between a metal ion and ligand from a DNA interaction study, probably a first of its kind.