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International Journal of Electrochemistry
Volume 2014, Article ID 186109, 8 pages
http://dx.doi.org/10.1155/2014/186109
Research Article

Action of Chicory Fructooligosaccharides on Biomimetic Membranes

1Biochemistry Laboratory, Federal University of Alfenas, R. Gabriel Monteiro da Silva, 700 Centro, 37130-000 Alfenas, MG, Brazil
2Materials Group Lab, Chemistry Institute, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil
3Integrative Animal Biology Laboratory, Institute of Biomedical Sciences, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil

Received 23 May 2014; Revised 17 October 2014; Accepted 19 October 2014; Published 16 November 2014

Academic Editor: Shen-Ming Chen

Copyright © 2014 A. F. Barbosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fructooligosaccharides from chicory (FOSC) are functional prebiotic foods recognized to exert several well-being effects in human health and animal production, as decreasing blood lipids, modulating the gut immune system, enhancing mineral bioavailability, and inhibiting microbial growth, among others. Mechanisms of actions directly on cell metabolism and structure are however little known. In this sense this work was targeted to investigate the interaction of FOSC with biomimetic membranes (liposomes and supported bilayer membrane; s-BLM) through cyclic voltammetry, impedance spectroscopy, spectrofluorimetry, and microscopy. FOSC was able to disrupt the membrane structure of liposomes and s-BLM from the onset of molecular pores induced on it. The mechanism of interaction of fructans with biomimetic membranes suggests hydrogen bonding between the polyhydroxylated structure of the oligosaccharides and the negative polar group of L--phosphatidylcholine (PC) present in both liposomes and s-BLM.