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International Journal of Forestry Research
Volume 2012, Article ID 318258, 9 pages
http://dx.doi.org/10.1155/2012/318258
Research Article

In Vitro Propagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components

Plant Tissue Culture Laboratory (CULTEV), Department of Basic Sciences, National University of Luján, C.C. 221, 6700 Luján, Argentina

Received 28 October 2011; Revised 17 January 2012; Accepted 17 January 2012

Academic Editor: Yousry El-Kassaby

Copyright © 2012 Ezequiel Enrique Larraburu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Handroanthus impetiginosus, pink lapacho, is a timber, ornamental, and medicinal tree. Experiments on the in vitro propagation of H. impetiginosus were conducted using nodal segments cultivated in both Murashige and Skoog salts with Gamborg vitamins (MSG) and Woody Plant Medium (WPM) with different concentrations of 6-benzylaminopurine (BA) and indole butyric acid (IBA). Morphogenic responses were differentially affected by salt compositions and their interactions with plant growth regulators in each micropropagation stage. According to response surface analysis, the optimum multiplication rate with 1 μM IBA ranged from 16.7 to 21.3 μM BA in WPM, and the inhibitors of endogenous auxins could increase multiplication rates. A pulse with 50 μM IBA in MSG produced 83% rooting with 3.2 roots per shoots and higher fresh and dry weights of shoots and roots. In the acclimatisation stage, 50% of plants survived after 1 year. This methodology optimised the culture media for the in vitro propagation of the H. impetiginosus clonal pool and could be applied to related species, several of which are categorised as vulnerable on the International Union for the Conservation of Nature Red List.