In Vitro Propagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components

Figure 1

In vitro propagation of Handroanthus impetiginosus. Initiation stage: (a) germinated seedlings. Multiplication stage: (b) initial explants, (c) shoot after 30 days culture. Rooting stage: (d) initial shoot, and (e) rooted shoot. Acclimatization stage: (f) plantlets in plastic tray for initial stage of acclimatization, (g) detail of plantlets inside plastic tray, (h) plant acclimatization in plastic pot after 90 days culture, and (i) acclimatized plant after one-year culture. Bars (a)–(e): 1 cm, (f)–(i): 3 cm.