In Vitro Propagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components
Table 3
Effect of benzylaminopurine (BA) and indole butyric acid (IBA) concentrations on in vitro shoot multiplication of Handroanthus impetiginosus on WPM basic medium after 30-day culture.
BA (μM)
IBA (μM)
MR1
Shoot length (mm)2
Internode length (mm)3
Shoot number4
0
0
1.75 ± 0.40
18.92 ± 7.37 ab
6.17 ± 2.07 abc
1.00 ± 0.00 d
5
0
1.72 ± 0.48
16.05 ± 3.78 bc
7.13 ± 2.00 a
1.28 ± 0.32 cd
5
1
2.44 ± 0.68
16.72 ± 6.03 bc
6.40 ± 2.20 ab
1.61 ± 0.42 bcd
5
2
2.54 ± 0.77
15.01 ± 7.37 bc
6.65 ± 2.01 ab
1.72 ± 0.49 bcd
5
4
2.24 ± 0.46
16.32 ± 5.04 bc
6.93 ± 1.63 ab
1.35 ± 0.33 cd
10
0
2.13 ± 0.50
15.22 ± 4.90 bc
5.66 ± 1.33 bcd
1.56 ± 0.45 bcd
10
1
3.58 ± 0.91
22.60 ± 7.26 a
7.22 ± 2.28 a
1.78 ± 0.48 bcd
10
2
1.94 ± 0.50
15.65 ± 4.64 bc
6.53 ± 1.52 ab
1.44 ± 0.38 bcd
10
4
1.51 ± 0.38
13.76 ± 3.85 cd
5.30 ± 1.47 cd
1.19 ± 0.25 cd
20
0
3.72 ± 1.15
16.19 ± 6.12 bc
6.38 ± 1.21 ab
3.00 ± 0.79 a
20
1
4.37 ± 0.98
18.86 ± 8.71 ab
6.43 ± 2.67 ab
1.94 ± 0.52 bc
20
2
3.30 ± 0.82
18.44 ± 6.79 ab
6.41 ± 1.31 ab
1.94 ± 0.54 bc
20
4
3.92 ± 1.28
16.72 ± 7.17 bc
6.90 ± 1.68 ab
2.17 ± 0.35 b
30
0
1.00 ± 0.00
10.31 ± 1.04 e
5.00 ± 0.00 de
1.00 ± 0.00 d
30
1
2.62 ± 0.65
19.76 ± 4.41 ab
6.30 ± 1.26 ab
1.44 ± 0.35 bcd
30
2
3.06 ± 1.02
15.26 ± 4.53 bc
5.15 ± 1.16 de
1.94 ± 0.63 bc
30
4
3.78 ± 1.22
11.90 ± 3.43 de
4.90 ± 0.96 de
3.40 ± 0.98 a
40
0
1.38 ± 0.38
10.40 ± 1.11 e
4.85 ± 0.47 de
1.10 ± 0.22 d
40
1
2.67 ± 0.74
16.53 ± 7.99 bc
6.38 ± 1.97 ab
1.39 ± 0.39 cd
40
2
1.94 ± 0.57
15.27 ± 4.15 bc
5.25 ± 0.53 cd
1.10 ± 0.15 d
40
4
2.81 ± 0.99
10.56 ± 2.00 e
4.48 ± 1.12 e
3.15 ± 1.03 a
WPM: Woody Plant Medium [10]. 1Multiplication rate: number of useful shoots obtained per initial shoot. 2Average of shoot length for all shoots obtained. 3Average of internode length for all shoot obtained. 4Initial and adventitious shoots. Different letters indicate significant differences between treatments by Tukey’s multiple range test (). Data are the averages of 2 replications of at least 56 shoots each.
