In Vitro Propagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components
Table 4
Effect of indole butyric acid (IBA) concentration and different formulations of basic culture media (1/2 WPM, 1/2 MSG) on root development parameters of in vitro rooting of Handroanthus impetiginosus with three days of auxin pulse induction, after 40-day culture.
Medium
IBA (μM)
Rooting percentage
Root number
Root length (mm)
Fresh root weight (mg)
Dry root weight (mg)
Root surface area1
1/2 WPM
0
42.65 ± 9.67 cd
2.15 ± 0.68 bc
16.14 ± 2.23 cdef
21.54 ± 0.90 cd
2.47 ± 0.86 cd
6.77 ± 0.59 ef
10
60.75 ± 12.92 bc
1.81 ± 0.51 bc
10.40 ± 2.71 f
17.30 ± 2.21 de
2.05 ± 1.68 d
7.69 ± 2.06 def
20
73.63 ± 21.16 abc
1.74 ± 0.47 bc
11.65 ± 3.41 ef
12.27 ± 2.75 e
2.30 ± 1.35 d
5.70 ± 2.51 f
30
57.77 ± 9.87 bc
2.39 ± 0.53 ab
19.87 ± 5.33 cde
22.86 ± 4.89 cd
3.36 ± 0.83 cd
11.14 ± 3.83 cde
40
60.99 ± 18.81 bc
2.07 ± 0.65 bc
11.66 ± 2.04 ef
21.04 ± 3.33 cd
2.67 ± 0.68 cd
13.11 ± 2.25 bcd
50
72.33 ± 12.71 abc
2.15 ± 0.50 bc
15.29 ± 3.19 def
17.92 ± 4.73 de
2.44 ± 2.01 cd
9.68 ± 2.29 def
1/2 MSG
0
25.13 ± 2.66 d
1.63 ± 0.47 bc
15.25 ± 2.28 def
24.45 ± 2.54 cd
2.62 ± 0.53 cd
19.39 ± 2.63 abc
10
58.68 ± 9.09 bc
1.80 ± 0.34 bc
51.56 ± 4.59 a
33.24 ± 6.81 bc
4.36 ± 0.82 bc
23.83 ± 4.13 a
20
60.64 ± 11.29 bc
1.79 ± 0.40 bc
37.35 ± 4.44 ab
30.13 ± 10.38 bcd
3.11 ± 1.13 cd
20.24 ± 6.63 ab
30
87.94 ± 3.69 a
1.37 ± 0.49 c
27.95 ± 7.64 bc
46.50 ± 7.94 ab
5.90 ± 2.26 ab
25.27 ± 6.75 a
40
47.50 ± 17.15 cd
2.04 ± 0.54 bc
22.17 ± 6.29 bcd
57.06 ± 9.89 a
2.83 ± 1.03 cd
24.72 ± 7.86 a
50
83.30 ± 21.99 ab
3.20 ± 0.59 a
28.07 ± 7.90 bc
66.21 ± 10.05 a
8.48 ± 3.33 a
29.27 ± 6.47 a
1/2 WPM: Woody Plant Medium [10] half concentration. 1/2 MSG: Murashige and Skoog [9] salts at half concentration with Gamborg vitamins [18]. 1Expressed as mg of saturated solution Ca(NO3)2 according to [19]. Different letters in the same column indicate significant differences between treatments by Tukey’s multiple range test (). Data are the averages of 3 replications of 20 shoots.
