A Simplified, Specific HPLC Method of Assaying Thiamine and Riboflavin in Mushrooms

Figure 4

HPLC chromatograms obtained in peak identification and spectral analysis of thiamine (B1) using photodiode array detector (PDA, 2996-Waters): (a) absorbance spectra of B2 from 190nm to 800nm, (b) reference standard, (c) purity plot of the B1 in standard solution, (d) reference standards solution, (e) purity plot of the B1 in test sample spiked with reference standards, and (f) test sample spiked with reference standards.

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