A Simplified, Specific HPLC Method of Assaying Thiamine and Riboflavin in Mushrooms

Figure 5

HPLC chromatograms obtained from Shimadzu prominence HPLC system with a UV-Vis detector (SPD-20AC) in method validation studies: (a) isocratic elution parameters, (b) diluting solution, (c) reference standards solution, (d) test sample spiked with reference standards, (e) LOD solution, and (f) test sample (Shitaki mushrooms).

(a)

(b)

(c)

(d)

(e)

(f)