Validation of a Simple and Robust Liebermann–Burchard Colorimetric Method for the Assay of Cholesterol in Selected Milk Products in Ghana

Table 3

Results showing precision of results from the developed method.

Precision parameters			Mean absorbance ± SD	RSD
Intra-assay precision	Outcome from 10 replicate determinations		95.97 ± 1.016	1.06%
	Triplicate determinations from three different concentrations	80%–0.032 mg/ml	96.88 ± 0.7221	0.75%
		100%–0.040 mg/ml	95.97 ± 1.016	1.06%
		120%–0.048 mg/ml	96.83 ± 0.7773	0.80%
Inter-assay precision	Same analyst	Day 1	94.25 ± 0.9697	1.03%
		Day 2	96.05 ± 1.516	1.58%
		Day 3	93.01 ± 1.314	1.41%
	Different analysts	Analyst 1	94.53 ± 0.8770	0.93%
		Analyst 2	95.91 ± 1.695	1.77%
		Analyst 3	93.42 ± 1.306	1.40%
			Acceptance criteria	<2%