Research Article | Open Access
Adelina Rogowska-Wrzesinska, Peter Mose Larsen, Anders Blomberg, Angelika Görg, Peter Roepstorff, Joakim Norbeck, Stephen John Fey, "Comparison of the Proteomes of Three Yeast Wild Type Strains: CEN.PK2, FY1679 and W303", International Journal of Genomics, vol. 2, Article ID 838532, 19 pages, 2001. https://doi.org/10.1002/cfg.94
Comparison of the Proteomes of Three Yeast Wild Type Strains: CEN.PK2, FY1679 and W303
Yeast deletion strains created during gene function analysis projects very often show drastic phenotypic differences depending on the genetic background used. These results indicate the existence of important molecular differences between the CEN.PK2, FY1679 and W303 wild type strains. To characterise these differences we have compared the protein expression levels between CEN.PK2, FY1679 and W303 strains using twodimensional gel electrophoresis and identified selected proteins by mass spectrometric analysis. We have found that FY1679 and W303 strains are more similar to each other than to the CEN.PK2 strain. This study identifies 62 proteins that are differentially expressed between the strains and provides a valuable source of data for the interpretation of yeast mutant phenotypes observed in CEN.PK2, FY1679 and W303 strains.
Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.