International Journal of Genomics

International Journal of Genomics / 2002 / Article

Review | Open Access

Volume 3 |Article ID 790536 | 6 pages |

Discovery of Single Nucleotide Polymorphisms and Mutations by Pyrosequencing

Received23 Oct 2001
Accepted27 Nov 2001


Comparative genomics, analyzing variation among individual genomes, is an area of intense investigation. DNA sequencing is usually employed to look for polymorphisms and mutations. Pyrosequencing, a real-time DNA sequencing method, is emerging as a popular platform for comparative genomics. Here we review the use of this technology for mutation scanning, polymorphism discovery and chemical haplotyping. We describe the methodology and accuracy of this technique and discuss how to reduce the cost for large-scale analysis.

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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