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Comparative and Functional Genomics
Volume 6 (2005), Issue 4, Pages 251-255
http://dx.doi.org/10.1002/cfg.479
Conference Review

Swine Genome Sequencing Consortium (SGSC): A Strategic Roadmap for Sequencing The Pig Genome

1Institute for Genomic Biology, University of Illinois, Urbana, IL, USA
2Department of Animal Sciences, University of Illinois, Urbana, IL, USA
3The Wellcome Trust Sanger Institute, Hinxton, UK
4Roslin Institute, Edinburgh, UK
5INRA-CEA, Jouy-en-Josas, France
6INRA-Toulouse, France
7Agricultural Research Service, Clay Center, NE, USA
8The Alliance for Animal Genomics, Bethesda, MD, USA
9Departments of Animal Sciences and Veterinary Pathobiology, University of Illinois, 382 Edward R. Madigan Laboratory, 1201 W. Gregory Dr., Urbana, IL 61801, USA

Received 22 February 2005; Revised 17 March 2005; Accepted 18 March 2005

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The Swine Genome Sequencing Consortium (SGSC) was formed in September 2003 by academic, government and industry representatives to provide international coordination for sequencing the pig genome. The SGSC’s mission is to advance biomedical research for animal production and health by the development of DNAbased tools and products resulting from the sequencing of the swine genome. During the past 2 years, the SGSC has met bi-annually to develop a strategic roadmap for creating the required scientific resources, to integrate existing physical maps, and to create a sequencing strategy that captured international participation and a broad funding base. During the past year, SGSC members have integrated their respective physical mapping data with the goal of creating a minimal tiling path (MTP) that will be used as the sequencing template. During the recent Plant and Animal Genome meeting (January 16, 2005 San Diego, CA), presentations demonstrated that a human–pig comparative map has been completed, BAC fingerprint contigs (FPC) for each of the autosomes and X chromosome have been constructed and that BAC end-sequencing has permitted, through BLAST analysis and RH-mapping, anchoring of the contigs. Thus, significant progress has been made towards the creation of a MTP. In addition, whole-genome (WG) shotgun libraries have been constructed and are currently being sequenced in various laboratories around the globe. Thus, a hybrid sequencing approach in which 3x coverage of BACs comprising the MTP and 3x of the WG-shotgun libraries will be used to develop a draft 6x coverage of the pig genome.