Global Transcriptional Analysis of Yeast Cell Death Induced by Mutation of Sister Chromatid Cohesin
Figure 1
Apoptotic cell death in mcd1-1 and pds5-1 mutants. WT, mcd1-1, and pds5-1 mutants were shifted from permissive temperature (C) to nonpermissive temperature (C) for 12 hours, then stained with (a) Annexin V, PI, and DAPI; (b) DAPI for nucleus and (c) Dihydrorhodamine 123 for ROS. (d) Quantitative measurement of ROS production of H2O2-treated cells, mcd1-1 and pds5-1 mutants at permissive, and nonpermissive temperature.
