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Comparative and Functional Genomics
Volume 2009 (2009), Article ID 380719, 11 pages
http://dx.doi.org/10.1155/2009/380719
Review Article

Reverse Genetics for Functional Genomics of Phytopathogenic Fungi and Oomycetes

1The MOA Key Laboratory of Molecular Plant Pathology, Department of Plant Pathology, China Agricultural University, Beijing 100193, China
2Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E2
3Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada S7N 5A8

Received 2 February 2009; Revised 30 May 2009; Accepted 7 July 2009

Academic Editor: J. Peter W. Young

Copyright © 2009 Vijai Bhadauria et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sequencing of over 40 fungal and oomycete genomes has been completed. The next major challenge in modern fungal/oomycete biology is now to translate this plethora of genome sequence information into biological functions. Reverse genetics has emerged as a seminal tool for functional genomics investigations. Techniques utilized for reverse genetics like targeted gene disruption/replacement, gene silencing, insertional mutagenesis, and targeting induced local lesions in genomes will contribute greatly to the understanding of gene function of fungal and oomycete pathogens. This paper provides an overview on high-throughput reverse genetics approaches to decode fungal/oomycete genomes.