Anaplasma phagocytophilum and Anaplasma marginale Elicit Different Gene Expression Responses in Cultured Tick Cells
Figure 1
Differential gene expression in A. phagocytophilum-infected I. scapularis ticks and cultured tick ISE6 cells. Real-time RT-PCR was done on uninfected and infected I. scapularis nymphs (three groups each of uninfected ticks, infected ticks (Gaillard strain; black bars) and infected ticks (Dawson strain; white bars) with 10 nymphs each) and uninfected and NY18 isolate-infected tick ISE6 cells (three independent cultures each; red bars). Bars represent the ratio between infected normalized Ct values/uninfected average normalized Ct values (+SD). The mRNA levels were normalized against tick -actin (ACT) and compared between infected and uninfected ticks and tick cells by Student’s -test (*).
