Research Article
Anaplasma phagocytophilum and Anaplasma marginale Elicit Different Gene Expression Responses in Cultured Tick Cells
Table 1
RT-PCR oligonucleotide primers and conditions for the characterization of the expression profiles of differentially expressed tick genes.
| Gene I | Upstream/downstream primer sequences | PCR annealing temperature |
| 1I1H6 | GGTACATGGAATCCGACTGC | 54°C | GTCCCCTTTTGCTTCGACTT | 1I3A8 | GACGCAAAACTTCCTTCGAG | 54°C | GCACTTTCCAAGAGCCTGAC | 1I3F5 | GCTTTCACGTTTTCGATGGT | 50°C | GGCAAAGATCCAAGACAAGG | 1I3H6 | GCCTAGGGAGGACGTCGTAG | 50°C | ACGTGGAACACATCGAGTCA | 1I4C6 | AATGCGAGACACTGGAGGAC | 50°C | AATCCAGGAATGTTGCCAAG | 1I4G12 | GACGGACCTTGTCCGACTAC | 53°C | ATTCCCTCCTTGTCCTGGAT | 1I5B9 | CGTCCCCTTCTGTGGAATTA | 53°C | TCATCGTTGTTCTGGTCTCG | 2I1C2 | GAGACCATCAAGTGGCTGGA | 53°C | CTTGGTGATGATGGGGTTG | 2I1F6 | CAACCCCAAGATCGTCAACT | 53°C | ACGCGTCCTTACGTTTCACT | 2IP10 | TCTTGCCGGTCAGAGTCTTT | 53°C | GAAGGCGAAAATTCAGGACA | 2I3A3 | TAAAACCCCTTTCCCCACTT | 53°C | GCACTCGAACCTAGCAAACC | 2I3A7 | TCGACTCTGTTCAGGAGGAAG | 53°C | GGTCCAAATGGCAGAGCAT | 2I3G1 | AGGAAGTGCACGATGATGG | 54°C | GGTTGGTTATCCTCTGGGAGA | 2I4F6 | CTTTCTTGCCGTGCTTCTTT | 53°C | GCTCAACTTCCTCGTCGTTC | UP8 | CCTCCCTCGCTAACCTCTCT | 54°C | ATCGTCACGGTCGAAGTAGC | U2A8 | GCTCATCGTCGCCAACAT | 54°C | GAGTTCCTCCGTCCAGCTC | C2E6 | GTAAAGCCCGCTCTCAAGAA | 53°C | CATTCGGGTTTGTCCACAG | C3B2 | GAGTAGTGCCCGTCTTCGAC | 53°C | AGGTGATGCTGCCCTTGTAG | C4G3 | AACTGCCTTGGAGTTGCAGT | 53°C | CTTGTGTCCCAGGTGGAAGT | C4B10 | GTTCTTCTAACGGCCACTGC | 53°C | AGTCTTTGGTGCAAGCGAGT | R1E12 | ATGTGAAGCTGAGGCCAAAC | 53°C | GGAATTCGATTAGCGTGGTC | R4G5 | CCTTCCCTGCAATGTCAAAT | 53°C | CACAAGTGGGCAATCAACAC | Beta actin | GAGAAGATGACCCAGATCA | 50°C | (AF426178) | GTTGCCGATGGTGATCACC | 16S rRNA | GACAAGAAGACCCTA | 42°C | (L34293) | ATCCAACATCGAGGT |
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Ds for I. scapularis genes are described herein and in de la Fuente et al. [9].
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