Research Article

Anaplasma phagocytophilum and Anaplasma marginale Elicit Different Gene Expression Responses in Cultured Tick Cells

Table 1

RT-PCR oligonucleotide primers and conditions for the characterization of the expression profiles of differentially expressed tick genes.

Gene I Upstream/downstream primer sequences PCR annealing temperature

1I1H6GGTACATGGAATCCGACTGC54°C
GTCCCCTTTTGCTTCGACTT
1I3A8GACGCAAAACTTCCTTCGAG54°C
GCACTTTCCAAGAGCCTGAC
1I3F5GCTTTCACGTTTTCGATGGT 50°C
GGCAAAGATCCAAGACAAGG
1I3H6GCCTAGGGAGGACGTCGTAG50°C
ACGTGGAACACATCGAGTCA
1I4C6AATGCGAGACACTGGAGGAC50°C
AATCCAGGAATGTTGCCAAG
1I4G12GACGGACCTTGTCCGACTAC53°C
ATTCCCTCCTTGTCCTGGAT
1I5B9CGTCCCCTTCTGTGGAATTA53°C
TCATCGTTGTTCTGGTCTCG
2I1C2GAGACCATCAAGTGGCTGGA53°C
CTTGGTGATGATGGGGTTG
2I1F6CAACCCCAAGATCGTCAACT53°C
ACGCGTCCTTACGTTTCACT
2IP10TCTTGCCGGTCAGAGTCTTT53°C
GAAGGCGAAAATTCAGGACA
2I3A3TAAAACCCCTTTCCCCACTT53°C
GCACTCGAACCTAGCAAACC
2I3A7TCGACTCTGTTCAGGAGGAAG53°C
GGTCCAAATGGCAGAGCAT
2I3G1AGGAAGTGCACGATGATGG54°C
GGTTGGTTATCCTCTGGGAGA
2I4F6CTTTCTTGCCGTGCTTCTTT53°C
GCTCAACTTCCTCGTCGTTC
UP8CCTCCCTCGCTAACCTCTCT54°C
ATCGTCACGGTCGAAGTAGC
U2A8GCTCATCGTCGCCAACAT54°C
GAGTTCCTCCGTCCAGCTC
C2E6 GTAAAGCCCGCTCTCAAGAA53°C
CATTCGGGTTTGTCCACAG
C3B2GAGTAGTGCCCGTCTTCGAC53°C
AGGTGATGCTGCCCTTGTAG
C4G3AACTGCCTTGGAGTTGCAGT53°C
CTTGTGTCCCAGGTGGAAGT
C4B10GTTCTTCTAACGGCCACTGC53°C
AGTCTTTGGTGCAAGCGAGT
R1E12ATGTGAAGCTGAGGCCAAAC53°C
GGAATTCGATTAGCGTGGTC
R4G5CCTTCCCTGCAATGTCAAAT53°C
CACAAGTGGGCAATCAACAC
Beta actinGAGAAGATGACCCAGATCA50°C
(AF426178)GTTGCCGATGGTGATCACC
16S rRNAGACAAGAAGACCCTA42°C
(L34293)ATCCAACATCGAGGT

Ds for I. scapularis genes are described herein and in de la Fuente et al. [9].