Anaplasma phagocytophilum and Anaplasma marginale Elicit Different Gene Expression Responses in Cultured Tick Cells

Table 2

Microarray analysis of gene expression profile in 𝐴.𝑚𝑎𝑟𝑔𝑖𝑛𝑎𝑙𝑒- and 𝐴.𝑝ℎ𝑎𝑔𝑜𝑐𝑦𝑡𝑜𝑝ℎ𝑖𝑙𝑢𝑚-infected and -uninfected tick ISE6 cells.

Probe ID^(a)	Description^(b)	A. marginale infection versus control		A. phagocytophilum infection versus control
		Fold change^(c)	SD^(d)	Fold change^(c)	SD^(d)
C4A10	No homolog found	6.249	0.000	2.208	0.294
R1A6	No homolog found	2.539	0.162	1.049	0.346
C3C5	[Genbank:L22271] internal transcribed spacer 1 (Ixodes dammini)	2.406	1.211	1.383	0.000
C4A8	No homolog found	2.239	0.165	1.188	0.442
R3A7	No homolog found	2.209	0.805	−1.384	0.562
C4G3	[Genbank:AAY66629] putative secreted salivary protein (Ixodes scapularis)	2.167	0.326	1.037	0.614
C2E6	[Genbank:ABB89211] troponin I protein (Rhipicephalus haemaphysaloides)	2.040	0.400	−1.068	0.442
C3C3	No homolog found	1.916	0.559	3.422	1.037
C4A1	No homolog found	1.857	0.000	2.121	0.517
R2A12	No homolog found	1.199	0.232	−2.219	0.450
C3A7	No homolog found	1.135	0.282	−3.028	0.141
C3D9	[Genbank:XP_791420] hypothetical protein (Strongylocentrotus purpuratus)	1.076	0.335	4.875	2.069
C3B2	[Genbank:BAE53722] aspartic protease (Haemaphysalis longicornis)	−1.311	0.330	−6.986	0.379
R2G1	No homolog found	−1.497	0.495	−2.086	0.826
R2D6	No homolog found	−1.538	0.309	−2.440	0.563
C3C11	No homolog found	−2.053	0.401	−2.477	0.488
C4D12	No homolog found	−2.066	0.547	−1.022	0.533
R4G5	[Genbank:AAP84098] ML domain-containing protein (Ixodes ricinus)	−2.066	0.161	1.020	0.266
C4C9	[Genbank:AAH56007] H13-prov protein (Xenopus laevis)	−2.070	0.270	1.081	0.689
C4G11	[Genbank:EAA09467] ENSANGP00000010016 (Anopheles gambiae)	−2.093	0.550	−1.193	0.598
C4G9	No homolog found	−2.095	0.402	−1.598	0.888
R3F5	[Genbank:AAY66764] putative secreted salivary protein (Ixodes scapularis)	−2.118	0.310	−1.037	0.320
R3G4	No homolog found	−2.292	0.259	1.090	0.415
R1F3	No homolog found	−2.339	0.570	−1.344	0.855
R1E12	[Genbank:NP_001119682] ribosomal protein L32 (Acyrthosiphon pisum)	−2.379	0.000	2.488	0.000
C3F10	[Genbank:AAM93633] putative secreted protein (Ixodes scapularis)	−2.386	0.545	−1.647	0.315
R3D4	No homolog found	−2.529	1.046	−2.377	0.518
C4D2	No homolog found	−2.702	0.860	1.043	0.972
R3F4	No homolog found	−2.928	0.298	1.174	0.396
C1H10	No homolog found	−3.341	0.307	−1.057	0.000
C4A4	No homolog found	−3.678	1.181	−1.430	0.331
C4E12	[Genbank:AAY66942] ribosomal protein S17 (Ixodes scapularis)	−3.964	0.822	1.430	0.993
C4B10	[Genbank:AAQ01562] von Willebrand factor (Ixodes ricinus)	−4.422	0.000	2.413	0.000

^(a)Probe ID (library plate and well) identifies sample (clone) in stock plates. ^(b)Description of the probe based on top (best) BLASTX alignment. ^(c)Fold change is the fold change of Lowess intensity-dependent normalized log2 ratio of valid background-corrected means averaged between valid replicates. Only entries displaying an expression change greater than 2-fold and 𝑃<.05 in either A. phagocytophilum or A. marginale infected cells are shown. Positive and negative values correspond to genes upregulated and downregulated in infected cells, respectively. ^(d)SD is the standard deviation determined from the normalized average log2 ratio but determined on data from valid spots only.