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Comparative and Functional Genomics
Volume 2010, Article ID 128297, 17 pages
Research Article

Computational Identification of Novel MicroRNAs and Their Targets in Vigna unguiculata

1Biology Department, Qingdao University of Science and Technology, Qingdao 266043, China
2Qingdao Academy of Agricultural Sciences, Qingdao 266100, China

Received 18 February 2010; Accepted 28 June 2010

Academic Editor: H. Heng

Copyright © 2010 Yongzhong Lu and Xiaoyun Yang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


MicroRNAs (miRNAs) are a class of endogenous, noncoding, short RNAs directly involved in regulating gene expression at the posttranscriptional level. High conservation of miRNAs in plant provides the foundation for identification of new miRNAs in other plant species through homology alignment. Here, previous known plant miRNAs were BLASTed against the Expressed Sequence Tag (EST) and Genomic Survey Sequence (GSS) databases of Vigna unguiculata, and according to a series of filtering criteria, a total of 47 miRNAs belonging to 13 miRNA families were identified, and 30 potential target genes of them were subsequently predicted, most of which seemed to encode transcription factors or enzymes participating in regulation of development, growth, metabolism, and other physiological processes. Overall, our findings lay the foundation for further researches of miRNAs function in Vigna unguiculata.