Research Article

Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm, Antheraea mylitta

Table 1

A list of transcripts showing similarity with known proteins.

ClusterNo. First HitAccession no. -valueLibrary information

(A) Protein synthesis

(1) Ribosomal proteins

Am[ ]1860S acidic ribosomal protein P2 [Spodoptera frugiperda]AAL62467 Silk gland
Am[ ]15Ribosomal protein S28 [Bombyx mori]NP_001037680 larvae (1d)

(2) Translation factors, t-RNA/amino acid synthases

Am[ ]10Glycyl-tRNA synthetase [Bombyx mori]NP_001040293 Silk gland
Am[ ]10Elongation factor 1 alpha [Papilio xuthus]BAG30769 Silk gland

(3) Chaperones, protein folding and secretion

Am[ ]20Hsp70 [Mythimna separata]ABY55233 larvae (1d)
Am[ ]15Heat shock protein hsp21.4 [Heliconius erato]ABS57447 Mixed

(4) RNA synthesis and processing

Am[ ]8Conserved hypothetical protein [Nasonia vitripennis]XP_001604727 larvae (1d)
Am[ ]4Transcriptional regulator, AraC family [Pseudoalteromonas atlantica T6c]YP_6604098 larvae (1d)
Am[ ]2Activating transcription factor [Bombyx mori]NP_001037041 Silk gland

(B) House keeping

(1) Energy metabolism

Am[ ]48Cytochrome c oxidase subunit III [Antheraea pernyi]NP_803442 Mixed
Am[ ]28cytochrome c oxidase subunit I [Antheraea yamamai]AAY78891 Mixed
Am[ ]11NADH:ubiquinone dehydrogenase, putative [Aedes aegypti]XP_001663929 larvae (1d)
Am[ ]8ATP synthase F0 subunit 6 [Antheraea pernyi]NP_803441 larvae (1d)
Am[ ]6Phosphoribosyl pyrophosphate synthetase [Bombyx mori]NP_001040481 larvae (1d)

(2) Structural proteins

Am[ ]23Troponin C 2 [Lonomia obliqua]AAV91416 larvae (1d)
Am[ ]7Flexible cuticle protein 12 precursorP45589 larvae (1d)
Am[ ]6Troponin I 1 [Lonomia obliqua]AAV91417 larvae (1d)

(3) Protein processing/degradation

Am[ ]2Protease [Helicoverpa armigera]ABU98623 larvae (1d)
Am[ ]3Ubiquitin [Antheraea yamamai]BAD05031 larvae (1d)

(4) Transportation

Am[ ]8Transport protein Sec61 gamma subunit [Bombyx mori]NP_001037675 Silk gland
Am[ ]4Vacuolar-type H+-translocating inorganic pyrophosphatase [Plasmodium chabaudi chabaudi]XP_7462280.2 larvae (1d)
A0149F1Fatty acid-binding protein 2 (FABP 2)P31417 larvae (1d)

(C) Other categories

(1) Detoxification

Am[ ]15Slucosyl/glucuronosyl transferases [Tribolium castaneum]XP_973188 Silk gland
Am[ ]9Carboxylesterase [Bombyx mori]NP_001040411 larvae (1d)
Am[ ]8Glutathione S-transferase theta [Antheraea pernyi]ACB36909 Silk gland

(2) Regulation

Am[ ]8Activated C kinase 1 receptor [Choristoneura fumiferana]AAZ296050Silk gland
Am[ ]5Cyclic nucleotide-specific phosphodiesterase long isoform [Plasmodium falciparum 3D7]ABS50256 larvae (1d)

(3) Immune related proteins/protease inhibitors

Am[ ]43Protease inhibitor 3 [Lonomia obliqua]AAV91425 Silk gland
Am[ ]17Hdd1-like protein [Trichoplusia ni]ABV68857 larvae (1d)
Am[ ]9Pacifastin light chain precursor [Pacifastacus leniusculus]AAC64661 larvae (1d)
Am[ ]7Serpin-like protein [Antheraea mylitta]ABG72716 Silk gland
(4) Silk proteins

Am[ ]127Seroin 2 [Antheraea mylitta]ABG72728 Silk gland
Am[ ]119Fibroin heavy chain [Antheraea yamamai]AAL02118 Silk gland
Am[ ]16Seroin 1 [Bombyx mori]NP_001037045 Silk gland

(5) Other functions

Am[ ]74Polyprotein [Kakugo virus]YP_015696 Silk gland
Am[ ]12Chemosensory protein 9 [Bombyx mori]NP_001037066 larvae (1d)

d indicates one day old larvae.