Comparative Global Gene Expression Profiles of Wild-Type Yersinia pestis CO92 and Its Braun Lipoprotein Mutant at Flea and Human Body Temperatures

Table 2

Transcripts altered in a Δlpp mutant of Y. pestis CO92 upon temperature shift from 26^∘𝐶 to 37^∘𝐶, compared to the wild-type strain, based on microarray analyses.

Yer ID	Gene Name	Gene Symbol	Function	Δlpp/WT
				FC
Cell envelope
YPO2394	major outer membrane lipoprotein	mlpA	lpp gene - cell envelope	−1138
YPO1527	putative membrane protein	—	Cell envelope	−1.7
YPO2417	putative membrane protein	—	Cell envelope	2.0
Metabolism
YPO2404	conserved hypothetical protein	—	Iron-sulfur cluster assembly scaffold protein	−2.2
YPO0408	putative aldolase	—	Energy metabolism	−115.9
y1235	putative ATP-binding protein of ABC transport system	—	Inorganic ion transport and metabolism	−1.7
y0015	malate synthase A	aceB	TCA cycle	−2.1
y0176	succinate-semialdehyde dehydrogenase	gabD	Gamma-aminobutyrate metabolism	1.5
Protein secretion and trafficking
YPO0502	similar to hemolysin-coregulated protein (Hcp)	—	Possible type VI secretion system effector	−52.8
YPO3275	Clp ATPase	htpM	Type VI secretion system clpB chaperone	−2.0
YPCD1.62	putative type III secretion regulatory protein	lcrQ/yscM	Blocks yop transcription	−5.3
YPO2597	sec-independent protein translocase protein	tatE	Protein and peptide secretion and trafficking	−1.7
Stressor response
YPO3643	major cold shock protein Cspa2	cspa2	Stress response	3.0
y0224	cold shock-like protein	cspI	Stress response	3.8
y0137	serine endoprotease	degQ	Protease/chaperon activated in response to stress	−1.9
YPO4085	heat shock protein	htpN/hslT	Protein folding and stabilization, stress response	−3.5
YPO3382	global stress requirement protein GsrA	htrA/degP	Protease/chaperon activated in response to stress	−2.9
YPO2897	DNA-binding transcriptional regulator IscR	iscR	Iron-sulfur cluster assembly, stress response	−2.0
YPO0238	mechanosensitive ion channel	mscL	Turgor regulator, activated in response to stress	−2.5
YPO2896	putative aminotransferase	nifS/iscS	Iron-sulfur cluster assembly, stress response	−1.9
YPO3969	universal stress protein B	uspB	Stress response	−1.9
Toxin production and resistance
YPO0337	similar to subtilase cytotoxin, subunit B	—	Putative toxin and probable virulence factor	−2.0
YPO0431	osmotically inducible protein Y	b4376	Toxin production and resistance	−55.7
YPO2333	quaternary ammonium compound-resistance protein	qacE	Toxin production and resistance	−4.1
Virulence factors
YPO2145	similar to the Bacillus subtilis stage V sporulation protein R	—	Involved in spore cortex formation in B. subtilis	−3.2
YPPCP1.05c	pesticin	pst	Bacteriocin that induces the formation of spheroplasts	−1.7
YPO3138	modulating protein YmoA (histone-like protein)	ymoA	Protein modification and repair, invasion	−2.2
Unknown functions
various genes (y1333, 1850, 3268, YPO0102, 2307, 3137, 3518, 3707, and 4064)				*

Functions were obtained from the CMR online database (http://cmr.jcvi.org) and from the literature. FC = fold-change, which was calculated as the ratio between the hybridization signal for that gene in the Y. pestis Δlpp mutant and WT bacteria (Δlpp/WT). Expression differences were deemed as statistically significant if the fold-change was ≥1.5 and 𝑃 value ≤.05. A negative sign (“−”) before the FC indicates down-regulation in the Y. pestis Δlpp mutant, relative to the WT strain. ∗FCs for genes with unknown functions (grouped together in the last line) ranged from −1.8 to −140.6.