Repertoire of SSRs in the Castor Bean Genome and Their Utilization in Genetic Diversity Analysis in Jatropha curcas

Table 1

SSRs used to detect polymorphisms in Jatropha curcas genotypes.

SSR locus	Repeat motif	Primer sequence	Location of repeat motif	No. of alleles detected	HO	HE	PIC
JM1	(TA)22	F: TTTGAGTGCTCCTATTTGGCTAGAA; R: CCAAATGACAAGTAGGCAGAACTTT	5′UTR	3	0.1832	0.1833	0.2
JM2	(TA)20	F: GAAATGAGAAGCCTTTACCCTCATT; R: AGAGGGAGAAGGGAGAAAGCAGT	5′UTR	2	0.5114	0.5115	0.4
JM3	(AT)20	F: TCATCGAATGGTAGAGAACTAATGG; R: TTAATTCGGATTCTGAGTCTTGAGG	5′UTR	2	0.2832	0.2832	0.3
JM4	(AT)29	F: ATTTGATACAGGAGCAGACCTCAAC; R: GTGGTGGTTATGGTGGTAAATTTGT	5′UTR	2	0.1166	0.1166	0.1
JM5	(TTA)22	F: GCAGAAACTCGGTAGAACTGTGAGT; R:GGCATAATCTACTGTTATCTCATCCC	5′UTR	3	0.2998	0.2999	0.2
JM6	(AT)24	F: CCAATCGGAGAGTGAAATAGAACAT; R: TCTCGAGTTTAAATCTTGGGTATGC	Intron	6	0.2149	0.2149	0.2
JM7	(TA)24	F: TGAGAGTGTTACAGAGAGTGTTGCTT; R: TGTTACTGCTGAAACATGGAATGAC	Intron	4	0.1499	0.1499	0.1
JM8	(TC)23	F: GAATTTAGAAGCCACATTTGAGACG; R: CCTATGTAACCCAAGAAAGACGATG	Intron	3	0.1499	0.1499	0.1
JM9	(TC)15	F: GTTAGAGAAGGCCAAATTGAAACTG; R: ACTTCATTACGTCGAGAGATATCGG	Exon	2	0.1832	0.1833	0.2
JM10	(GAA)15	F: TGGAAGACGAATACTATGACGATGA; R: CAGGTGCTACTTCTTCTTCTTCAGG	Exon	2	0.1149	0.115	0.1
JM11	(CAT)12	F: GCATGCAAACCCTGAATTATGTACT; R: GCTGCTGACTCTGTTTCTCCTTCTA	Exon	2	0.2149	0.2149	0.2
JM12	(TA)33	F: TTGGCTCATAATAACTCCTCAAAGC; R: GCGAGTGCTGTCTAAAGCCTAATTT	Non genic	2	0.1149	0.115	0.1
JM13	(AT)25	F: GTCAGTACCTACAAGCTGCCTTCAT; R: GCCTTTGGATGAACCTATTCACATA	Non genic	5	0.1832	0.1833	0.2
JM14	(AT)34	F: GTTTGGCGATGAGCTAATTGAGATT; R: GGCTCGAACCTTTCTGATCTAATGT	Non genic	2	0.2732	0.2732	0.2
JM15	(TA)42	F: TGTAGATAGCCTTAGCTGTGCATTG; R: GTACTCTCGAGGGAGTTGATTGTGT	Non genic	2	0.5397	0.5398	0.5
JM16	(TA)38	F: TTGGCTCATAATAACTCCTCAAAGC; R: GCGAGTGCTGTCTAAAGCCTAATTT	Non genic	2	0.4481	0.4481	0.4

JM: Jatropha microsatellite; F: forward; R: reverse.