Cytoplasmic Ribonucleoprotein Foci in Eukaryotes: Hotspots of Bio(chemical)Diversity

Figure 1

Colocalization of eIF4E with components of PBs and SGs shows a diversity of cytoplasmic foci quality. The experiment shows that, in every case, the granules contain both components or either one or the other in different quantities. This would represent intermediates or different forms of eIF4E-containing foci. Drosophila melanogaster S2 cells were transfected with proteins fusion CFP-Lsm-1 or CFP-Me31B. GW182, eIF4E, and Rox8 (the TIA-1 ortholog in Drosophila) were revealed using antibodies against GW182, anti-V5, and anti-TIA-1, respectively. In the bottom panel (row 4), the cells were prestressed with arsenite for 30 minutes.