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Comparative and Functional Genomics
Volume 2012, Article ID 504292, 7 pages
http://dx.doi.org/10.1155/2012/504292
Review Article

Cytoplasmic Ribonucleoprotein Foci in Eukaryotes: Hotspots of Bio(chemical)Diversity

1Centro Regional de Estudios Genómicos, Universidad Nacional de La Plata, CP 1888 Florencio Varela, Argentina
2Departamento de Ciencias Básicas y Experimentales, Universidad Nacional del Noroeste de Buenos Aires, Avenida Calchaquí 5900, CP 1888 Buenos Aires, Pergamino, Argentina

Received 1 February 2012; Accepted 22 March 2012

Academic Editor: Greco Hernández

Copyright © 2012 Carla Layana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Colocalization of eIF4E with components of PBs and SGs shows a diversity of cytoplasmic foci quality. The experiment shows that, in every case, the granules contain both components or either one or the other in different quantities. This would represent intermediates or different forms of eIF4E-containing foci. Drosophila melanogaster S2 cells were transfected with proteins fusion CFP-Lsm-1 or CFP-Me31B. GW182, eIF4E, and Rox8 (the TIA-1 ortholog in Drosophila) were revealed using antibodies against GW182, anti-V5, and anti-TIA-1, respectively. In the bottom panel (row 4), the cells were prestressed with arsenite for 30 minutes.

Relationship among active polysomes and PBs. The recruitment of active polysomes to PB implies the removal from the mRNA of the translation factors by translational repressors. Some of them have been demonstrated to interact with eIF4E in vivo (rck/p54 and eIF4E-T, [20]). They further interact and/or recruit the enhancers of decapping Lsm1-7 or the miRNA-related protein GW182 to form the PB. Later on, they assemble the decapping and degradation enzymes and/or the proteins required for silencing and storage into PB. All the intermediate steps of this process can represent different populations of granules coexisting in the cell and visible with different morphology that might reflect a variety of components and/or diverse stoichiometry.

  1. Supplementary Video