PASSIOMA: Exploring Expressed Sequence Tags during Flower Development in Passiflora spp.
Figure 5
In situ expression patterns of putative P. edulis genes involved in flower development. The hybridization signal is observed as a pink-red precipitate. (a–e) and (h) show longitudinal sections though early stages of P. edulis flower meristems. (f, g, i, and j) show transversal sections of 4 mm long P. edulis flower buds. (a) the hybridization signal of PACEPE3008B09, a putative KANADI ortholog, can be detected throughout the floral meristem and in the adaxial side of the bracts. As floral organ primordia are formed, the expression is confined to the adaxial side (not shown). (b) the expression of PACEPE3005G07, a putative YABBY ortholog, is restricted to the floral meristem dome and to the adaxial side of all floral organ primordia. (c) the hybridization signal of PACEPE3023H04, a putative TCP9 ortholog, is restricted to the dome of both floral and axillary vegetative meristems. (d) transcripts of PACEPE3007D03, a putative SEPALLATA ortholog, are detected not only in the floral meristem, but also in the tendril primordium. (e) the hybridization signal of PACEPE3002E10, a putative PISTILLATA ortholog, is restricted to the group of cells in whorls 3 (petal primordia) and 4 (stamen primordia, arrowheads), but later in development (f, g), it is also detected in the group of cells that originate the corona filaments (g) is a magnification of the insert shown in the lower right corner in (f). (h) transcripts of PACEPE3011A12, a putative AGAMOUS ortholog, are detected in the floral meristem dome early in development, but later in development (i, j), it is also detected in the group of cells that originate the corona filaments ((j) is a magnification of the insert shown in the lower right corner in (i), arrows point to primordia of the corona filaments). ab: abaxial side; ad: adaxial side; an: anther; br: bract primordium; co: corona primordia; fm: floral meristem; lp: leaf primordium; ov: ovary; pe: petal primordium; se: sepal primordium; tp: tendril primordium; vm: vegetative meristem. Bars: (a–e and h): 200 μm; (g): 20 μm; (j): 60 μm.
