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International Journal of Genomics
Volume 2013, Article ID 269191, 9 pages
Research Article

De Novo Transcriptome Assembly and Differential Gene Expression Profiling of Three Capra hircus Skin Types during Anagen of the Hair Growth Cycle

The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region 010021, China

Received 27 December 2012; Accepted 3 April 2013

Academic Editor: Soraya E. Gutierrez

Copyright © 2013 Teng Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure 1: Schematic representations of the gene expression profile in three goat skin libraries. (A) Transcript abundance levels in the belly and back skin library (BL vs BK). (B)Transcript abundance levels in the belly and side of body skin library (BL vs BS). (C) Transcript abundance levels in the back and side of body skin library (BK vs BS).

Table 1: Validation of ten putative full-length CDSs was conducted by using RT-PCR and Sanger sequencing.

Table 2: Validation of ten transcripts which were previously demonstrated specific to hair cycling by using RT-PCR and assembly annotation.

Table 3: Codon usage of 23,039 predicted CDSs of the Cashmere goat.

Table 4: Functional annotation and gene expression patterns from BL, BK and BS skin RNA-Seq libraries of the Cashmere goat during anagen phase.

Table 5: KOG enrichment analysis of 6333 consistently differentially expressed genes compared with transcriptome background.

Table 6: Examining differentially expressed genes by using qRT-PCR and direct comparison with RNA-Seq.

  1. Supplementary Figure 1
  2. Supplementary Table 1
  3. Supplementary Table 2
  4. Supplementary Table 3
  5. Supplementary Table 4
  6. Supplementary Table 5
  7. Supplementary Table 6