The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori)
Figure 2
(a) The expression and purification of recombinant BmBras2 were analyzed by SDS-PAGE. (b) The expression and purification of recombinant BmBras2 were analyzed by Western blotting. M, protein mass marker; 1, purified recombinant protein by Ni-NTA superflow cartridges; 2, the lysate of E. coli Rosetta with pET-28a (+)-BmBras2 without induction; 3, the lysate of E. coli Rosetta with pET-28a (+)-BmBras2 after IPTG induction. (c) The lysate of Bm5 was analyzed by SDS-PAGE. (d) The Bm5 cells were collected for western bolt with anti-BmBras2. (Bm5 cells were seeded at cells per flask in culture flask and cultured for three days at 37°C in a 5% CO2 incubator.) M, protein mass marker; 1, the lysate of Bm5 cells.
