Research Article

The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori)

Figure 4

Distribution of BmBras2 in different fifth instar larvae tissues. (a) tissue distributions of BmBras2 by western blotting; 1, head; 2, intestine; 3, epidermis; 4, silk gland; 5, fat body; 6, malpighian tubule; 7, ovaries; 8, testis; 9, purified rBmBras2. (b) tissue distributions of BmBras2 analyzed by real-time PCR. Sg, silk gland; Fb, fat body; Mt, malpighian tubule. The relative expression level was calculated by using here = (CT, BmBras2-CT, 18S rRNA) for different tissues and (CT, BmBras2-CT, 18S rRNA) fat body.
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