Molecular Analysis of Twist1 and FGF Receptors in a Rabbit Model of Craniosynostosis: Likely Exclusion as the Loci of Origin
Figure 2
SNP discovery and genotyping of Twist1 in craniosynostotic rabbits. We identified a SNP within the Twist1 coding region, a silent mutation, that segregates independently of craniosynostosis within our colony, ruling out Twist1 as the causative agent. ((a)–(c)) Sequencing traces representing C/C (a), T/T (b), or C/T (c) genotypes from PCR using CS genomic DNA. (d) Results of sequencing 22 randomly selected CS animals; six animals were C/C genotype, three animals were T/T, and 13 animals were C/T.
