Differential Expression of Myogenic Regulatory Factor Genes in the Skeletal Muscles of Tambaqui Colossoma macropomum (Cuvier 1818) from Amazonian Black and Clear Water

Table 1

Primer sequences used for RT-PCR and qRT-PCR reactions of myod, myf5, myogenin (MyoG), and mrf4 amplifications.

Primers	Sequences (5′-3′)	Genes	Design
MDLE	CTAACCAGAGGCTGCCHAAG	myod	Ictalurus furcatus
MDRI	CATGCCATCWGAGCAGTTGG	myod	Ostariophysi
Myf5FB	GCACGTGCGGGCTCCTG	myf5	Danio rerio
Myf5RE	GGACAAACACTGCAAACTGG	myf5	D. rerio
MGLG	TGGAGCTTTTYGAGACCAAC	myog	Cyprinus carpio
MGRF	AGATTGGCTTGCTCCGAAGA	myog	I. furcatus
MRF4-3	TACCAAGGAAATGACAGCCCTCCA	mrf4	Sternopygus macrurus
MRF4-4	TCAACAATTGAGGAGAGGCGACGA	mrf4	S. macrurus
TambMyoD1	GCCTGCAGGGACTCGATGTA	myod	C. macropomum*
TambMyoD2	GCTGCCCAAGGTGGAGATC	myod	C. macropomum*
Myf5RT2f	GGCGCAGGCTGAAGAAGGTCAA	myf5	C. macropomum*
Myf5RT2r	GCGCCATCCAGTACATTGAGA	myf5	C. macropomum*
TambMyoG1	GCGCCATCCAGTACATTGAGA	myog	C. macropomum*
TambMyoG2	GCTCATGTTCCTGCTGGTTGA	myog	C. macropomum*
F4RT2f	GTGCTGCGTGTTGATCTGCGT	mrf4	C. macropomum*
F4RT1r	AGGGGCTCAATGGCCTGCTT	mrf4	C. macropomum*

∗Primers used for RT-qPCR amplifications.