Extensive Differences in Antifungal Immune Response in Two Drosophila Species Revealed by Comparative Transcriptome Analysis

Figure 5

Neighbor-joining phylogenetic tree of Drosophila Defensin genes with antifungal Defensin genes of sand fly (Phlebotomus duboscqi) and mosquito (Anopheles gambiae). Amino acid sequences of the mature peptide were aligned by CLUSTAL W [12] and the phylogenetic tree was reconstructed with the Poisson model by MEGA5 [13]. For each Defensin gene, abbreviated four-letter species code (Dmel: Drosophila melanogaster, Dsec: D. sechellia, Dsim: D. simulans, Dere: D. erecta, Dyak: D. yakuba, Dana: D. ananassae, Dper: D. persimilis, Dpse: D. pseudoobscura, Dgri: D. grimshawi, Dmoj: D. mojavensis, Dvir: D. virilis, Dwil: D. willistoni, Pdub: Phlebotomus duboscqi and Agam: Anopheles gambiae) with Gene ID or Uniprot ID in parenthesis is shown as an operational taxonomic unit. The Defensins genes of D. melanogaster and D. virilis were indicated by bold face. The number along each branch is the bootstrap value computed by 1,000 bootstrap replicates.