Identification of Putative Ortholog Gene Blocks Involved in Gestant and Lactating Mammary Gland Development: A Rodent Cross-Species Microarray Transcriptomics Approach
Figure 6
Gene expression profiles of the putative transcriptional factors identified by microarray using qRT-PCR. Quantitative real time-PCR was performed as in Experimental Procedures, and the calculated mean log ratio for each gene in their corresponding developmental stage was determined. A positive value indicates a greater mRNA abundance in a developmental stage than in the control group, whereas a negative value indicates a lower mRNA abundance in a developmental stage than in the control group. Bars represent data obtained by qRT-PCR (y-axis), whereas the x-axis represents the physiological time point. The average fold change relative to time-matched virgin controls for three animals per group is shown. Genes are indicated by their official gene symbols. Data are presented as the mean of three independent experiments, each performed in triplicate. Bars represent the average (±SD) fold change. Different letters indicate statistically significant differences between developmental stages (). Note that the scales of the y-axis vary among genes. ND: not detected.
