Research Article

Identification of Cassava MicroRNAs under Abiotic Stress

Figure 6

Validation of 5 predicted cassava miRNAs. (a) Mean cycle threshold (Ct) values showing the expression levels of the selected miRNAs under normal conditions; Ct values indicate the number of PCR cycles at which the amplification signal crosses a fixed threshold; lower Ct values correspond to higher expression levels. Error bars correspond to the standard deviation (SD) of Ct values ( ). (b) Comparison of the relative expression levels of the selected miRNAs under heat and drought treatments normalized to values in (a) using the method. 18s rRNA was chosen as an endogenous control; error bars correspond to the normalized SD of values ( ). *: Mann-Whitney value ; RT: Room temperature.
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(a)
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(b)