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International Journal of Genomics
Volume 2014, Article ID 381501, 7 pages
http://dx.doi.org/10.1155/2014/381501
Research Article

Identification of Salt-Stress-Induced Genes from the RNA-Seq Data of Reaumuria trigyna Using Differential-Display Reverse Transcription PCR

1Key Laboratory of Herbage & Endemic Crop Biotechnology and College of Life Sciences, Inner Mongolia University, Hohhot 010021, China
2School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia

Received 5 August 2014; Revised 27 October 2014; Accepted 10 November 2014; Published 26 November 2014

Academic Editor: Qu Zhang

Copyright © 2014 Zhen-hua Dang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Gahlan, H. R. Singh, R. Shankar et al., “De novo sequencing and characterization of Picrorhiza kurrooa transcriptome at two temperatures showed major transcriptome adjustments,” BMC Genomics, vol. 13, no. 1, article 126, 2012. View at Publisher · View at Google Scholar · View at Scopus
  2. Y. Zhou, F. Gao, R. Liu, J. Feng, and H. Li, “De novo sequencing and analysis of root transcriptome using 454 pyrosequencing to discover putative genes associated with drought tolerance in Ammopiptanthus mongolicus,” BMC Genomics, vol. 13, no. 1, article 266, 2012. View at Publisher · View at Google Scholar · View at Scopus
  3. G. Liu, W. Li, P. Zheng et al., “Transcriptomic analysis of “Suli” pear (Pyrus pyrifolia white pear group) buds during the dormancy by RNA-Seq,” BMC Genomics, vol. 13, no. 1, article 700, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. Y. Ma, Flora Intramongolica (Tomus 3), Typis Intramongolicae Popularis, Hohhot, China, 1989.
  5. Y. Zhao, Vascular Plants in Ordos Plateau, Inner Mongolia University Press, Hohhot, China, 2006.
  6. Y. Xue and Y. Wang, “Study on characters of ions secretion from Reaumuria trigyna,” Journal of Desert Research, vol. 28, pp. 437–442, 2008. View at Google Scholar
  7. Y. Xue, Y. Wang, and T. Wang, “Physiological and biochemical mechanisms of an endemic halophyte Reaumuria trigyna Maxim. under salt stress,” Acta Botanica Boreali-Occidentalia Sinica, vol. 32, pp. 136–142, 2012. View at Google Scholar
  8. Z.-H. Dang, L.-L. Zheng, J. Wang et al., “Transcriptomic profiling of the salt-stress response in the wild recretohalophyte Reaumuria trigyna,” BMC Genomics, vol. 14, no. 1, article 29, 2013. View at Publisher · View at Google Scholar · View at Scopus
  9. X. Wang, L. Chang, Z. Sun, and H. Ma, “Characterization of genes expressed in response to cadmium exposure in the earthworm Eisenia fetida using DDRT-PCR,” Ecotoxicology and Environmental Safety, vol. 73, no. 6, pp. 1214–1220, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. S. F. Altschul, T. L. Madden, A. A. Schäffer et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,” Nucleic Acids Research, vol. 25, no. 17, pp. 3389–3402, 1997. View at Publisher · View at Google Scholar · View at Scopus
  11. A. Bräutigam and U. Gowik, “What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research,” Plant Biology, vol. 12, no. 6, pp. 831–841, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. R. Ekblom and J. Galindo, “Applications of next generation sequencing in molecular ecology of non-model organisms,” Heredity, vol. 107, no. 1, pp. 1–15, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Tunnacliffe and M. J. Wise, “The continuing conundrum of the LEA proteins,” Naturwissenschaften, vol. 94, no. 10, pp. 791–812, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. X. J. Li, M. F. Yang, H. Chen, L. Q. Qu, F. Chen, and S. H. Shen, “Abscisic acid pretreatment enhances salt tolerance of rice seedlings: proteomic evidence,” Biochimica et Biophysica Acta—Proteins and Proteomics, vol. 1804, no. 4, pp. 929–940, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. K. Aghaei, A. A. Ehsanpour, A. H. Shah, and S. Komatsu, “Proteome analysis of soybean hypocotyl and root under salt stress,” Amino Acids, vol. 36, no. 1, pp. 91–98, 2009. View at Publisher · View at Google Scholar · View at Scopus
  16. H. Zhang, B. Han, T. Wang et al., “Mechanisms of plant salt response: insights from proteomics,” Journal of Proteome Research, vol. 11, no. 1, pp. 49–67, 2012. View at Publisher · View at Google Scholar · View at Scopus
  17. J. E. Dombrowski, “Salt stress activation of wound-related genes in tomato plants,” Plant Physiology, vol. 132, no. 4, pp. 2098–2107, 2003. View at Publisher · View at Google Scholar · View at Scopus
  18. T. Bureau, K. C. Lam, R. K. Ibrahim, B. Behdad, and S. Dayanandan, “Structure, function, and evolution of plant O-methyltransferases,” Genome, vol. 50, no. 11, pp. 1001–1013, 2007. View at Publisher · View at Google Scholar · View at Scopus
  19. P. Liang and A. B. Pardee, “Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction,” Science, vol. 257, no. 5072, pp. 967–971, 1992. View at Publisher · View at Google Scholar · View at Scopus
  20. Y.-J. Sung and R. B. Denman, “Use of two reverse transcriptases eliminates false-positive results in differential display,” BioTechniques, vol. 23, no. 3, pp. 462–468, 1997. View at Google Scholar · View at Scopus