Research Article
Functional Expression Study of Igf2 Antisense Transcript in Mouse
Table 2
Primer pairs and probes used in TaqMan qPCR experiments. Forward (F) and reverse (R) primers are indicated.
| | Primer/probe | Sequence () | Product |
| | F_Actb | GCTTCTTTGCAGCTCCTTCGT | | | R_Actb | GCGCAGCGATATCGTCATC | | | Probe_Actb | CCGGTCCACACCCGCCACC | 71 bp | | F_Gapdh | CGGCCGCATCTTCTTGTG | | | R_Gapdh | TACGGCCAAATCCGTTCAC | | | Probe_Gapdh | AGTGCCAGCCTCGTCCCGTAGACA | 78 bp | | F_Igf2 V1 | CCGGCTTCCAGGTACCAAT | | | R_Igf2 V1 | GCAGCGATGCAGCACAAG | | | Probe_Igf2 V1 | ATGTTGGTGCTTCTCATCTCTTTGGCCTT | 90 bp | | F_Igf2 V2 | GCCCTTCTCCTCCGATCCT | | | R_Igf2 V2 | ATGAGAAGCACCAACATCGACTT | | | Probe_Igf2 V2 | CGACCTTCGGCCTTGTGGTACCAA | 98 bp | | F_Igf2 V3 | CCAGCCTTTTCCTGTCTTCATC | | | R_Igf2 V3 | CCATTGGTACCTGAAGTTGGGTAA | | | Probe_Igf2 V3 | TTCCAGCCCCAGCGGCCTC | 68 bp | | F_Igf2 P0 | TGACTCCCCGGTCCTCTTT | | | R_Igf2 P0 | TGTTTTAATTACTCGTCCCCTGTACTC | | | Probe_Igf2 P0 | TCCACCGTCCGGGAACTTCAGG | 84 bp | | F_Igf2 Pm | TCGGAGAGAAGTAGGGTACCAATG | | | R_Igf2 Pm | GGCGAAGGCCAAAGAGATG | | | Probe_Igf2 Pm | AAGTCGATGTTGGTGCTT | 77 bp | |
F_Igf2as MGB | TGCACCAAACTGATCAACACAA | | |
R_Igf2as MGB | AAAGGCAGGGTTCCAGATGA | | |
Probe_Igf2as MGB | CACACCAAAAATTCTTTC | 62 bp |
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