Genetic Variations of Cytokines and Cytokine Receptors in Psoriasis Patients from China

Table 1

Primer sequences, PCR product lengths, restriction endonucleases, and restriction patterns for the PCR-RFLP analyzed SNPs.

SNP	Gene	Position of SNP in genomic sequence	Forward primer^a	Reverse primer	Anneal temperature (°C)	Product length (bp)	Restriction endonucleases	Fragments of frequent allele genotype (bp)	Fragments of heterozygous genotype (bp)	Fragments of rare allele genotype (bp)	Reference
rs2243250	IL4	132009154:C/T	TAAACTTGGGAGAACATGGT	TGGGGAAAGATAGAGTAATA	49	195	AvaII	195	22 + 173 + 195	22 + 173	[1]
rs1800872	IL10	206946407:A/C	AGGTGATGTAATATCTCTGT	TAAATATCCTCAAAGTTCC	57	303	RsaI	65 + 238	65 + 238 + 303	303	[2]
rs3212227	IL12B	158742950:A/C	TTCTATCTGATTTGCTTTA	TGAAACATTCCATACATCC	51	233	TaqI	233	68 + 165 + 233	68 + 165	[3]
rs1800925	IL13	131992809:C/T	GTCGCCTTTTCCTGCTCTTCCCGC	GGAATCCAGCATGCCTTGTGAGG	65	247	Bsh1236I	23 + 224	23 + 224 + 247	247	[4]
rs20541	IL13	131995964:C/T	TAGGCTGAAGACGGGCAGCA	AAGAAACTTTTTCGCGAGGGCC	63	199	MspI	22 + 177	22 + 177 + 199	199	[5]
rs56245420	IL15	142873720:A/T	TTTCTGTTATTAACAAACATCACTCTG	CAACACTTGTACATATTTTTATTCAATAT	54	274	SspI	27 + 247	27 + 247 + 274	274	This study

aMismatch is shown in bold and underlined font.