Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae

Table 1

Media composition used for metabolic footprinting experiment.

	Group	Formula	Common name	MW	g/L
Yeast nitrogen base	Nitrogen source	(NH4)2SO4	Ammonium sulphate	132.13952	5.0198
	Vitamins	C10H16N2O3S	Biotin	244.31064	0.00002
		C18H32CaN2O10	Calcium pantothenate	476.53208	0.002
		C19H19N7O6	Folic acid	441.39746	0.002
		C6H12O6	Inositol	180.15588	0.01
		C6H5NO2	Niacin	123.1094	0.0004
		C7H7NO2	p-Aminobenzoic acid	137.13598	0.0002
		C8H11NO3⋅HCl	Pyridoxine hydrochloride	205.63878	0.00056
		C17H20N4O6	Riboflavin	376.3639	0.0002
		C12H17ClN4OS⋅HCl	Thiamin hydrochloride	337.26852	0.0004
	Trace elements	H3BO3	Boric acid	61.83302	0.05
		CuSO4	Copper sulphate	159.6086	0.00004
		KI	Potassium iodide	166.00277	0.0001
		FeCl3	Ferric chloride	162.204	0.0002
		MnSO4	Manganese sulphate	151.000645	0.0004
		Na2MoO4	Sodium molybdate	205.9171386	0.0002
		ZnSO4	Zinc sulphate	161.4716	0.0004
	Salts	KH2PO4	Potassium phosphate monobasic	136.085542	0.85
		K2HPO4	Potassium phosphate dibasic	174.175902	0.15
		MgSO4	Magnesium sulphate	120.3676	0.5
		NaCl	Sodium chloride	58.44276928	0.01
		CaCl2	Calcium chloride	110.984	0.1
Metabolite cocktail	Amino acids (all L-form)	C6H14N4O2	Arginine	174.20096	0.1742
		C4H6NO4K	Aspartate (monopotassium salt)	171.19304	0.1712
		C5H8NNaO4	Glutamate (monosodium salt)	169.1110893	0.1691
		C6H9N3O2	Histidine	155.15456	0.1552
		C6H13NO2	Leucine	131.17292	0.1312
		C6H14N2O2	Lysine (hydrochloride)	146.18756	0.1462
		C5H11NO2S	Methionine	149.21134	0.1492
		C3H7NO3	Serine	105.09258	0.1051
		C4H9NO3	Threonine	119.11916	0.1191
		C11H12N2O2	Tryptophan	204.22518	0.02042
		C5H11NO2	Valine	117.14634	0.1051
	Organic acids	C6H8O7	Citrate	192.12352	0.2101
		C4H2O4Na2	Fumarate (disodium salt)	160.0358186	0.16
		C4H6O5	Malate	134.08744	0.1341
		C3H4O3	Pyruvate	88.06206	0.0881
		C4H4Na2O4	Succinate (disodium salt)	162.0516986	0.2701
	Base	C4H5N3O	Cytosine	111.102	0.1111
		C4H4N2O2	Uracil	112.08676	0.1121