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International Journal of Genomics
Volume 2015, Article ID 132720, 6 pages
http://dx.doi.org/10.1155/2015/132720
Research Article

Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer

1Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2Students Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
3HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Received 5 March 2015; Accepted 31 May 2015

Academic Editor: Giulia Piaggio

Copyright © 2015 Reza Mahmoudi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recent studies have shown that polymorphisms in leptin and leptin receptor genes are associated with increased risk for breast cancer. This study aimed at investigating -2548 G/A polymorphism in leptin gene and Q223R polymorphism in leptin receptor gene in patients with breast cancer. The study included 45 women with breast cancer and 41 healthy women. PCR-RFLP was used to determine the genotype of the subjects in terms of -2548 G/A polymorphism in leptin gene and Q223R polymorphism in leptin receptor gene. Serum levels of leptin were also measured by ELISA. For -2548 G/A polymorphism, the genotypes were homozygous AA (OR = 1.13; ) and heterozygous GA (OR = 0.41; ) and for Q223R polymorphism, the genotypes were homozygous RR (OR = 6.7; ) and heterozygous QR (OR = 8.3; ). The mean serum level of leptin was 33.22 ± 21.35 ng/mL in patients and 29.49 ± 23.27 ng/mL in the normal participants (). Although, despite the magnitude of the associations, the results suggested no statistically significant contribution of -2548 G/A polymorphism (in leptin gene), Q223R polymorphism (in leptin receptor gene), and serum leptin levels in predicting the risk of breast cancer, further studies with larger sample size are suggested.