Research Article

Phylogenomic and Molecular Demarcation of the Core Members of the Polyphyletic Pasteurellaceae Genera Actinobacillus, Haemophilus, and Pasteurella

Figure 2

A partial sequence alignment of a 3′-nucleotidase showing a 1-amino-acid insertion identified in all members of Actinobacillus sensu stricto. This insertion was not found in the homologues from any member of the genus Actinobacillus that was not part of the “sensu stricto” clade or any other member of the Gammaproteobacteria. Sequence information for a representative subset of the family Pasteurellaceae and the class Gammaproteobacteria is shown, but unless otherwise indicated, similar CSIs were detected in all members of the indicated group and not detected in any other bacterial species in the top 250 BLAST hits. The dashes (-) in the alignments indicate identity with the residue in the top sequence. GenBank identification (GI) numbers for each sequence are indicated in the second column. Sequence information for other CSIs specific to Actinobacillus sensu stricto are presented in Supplemental Figures  1-2 and their characteristics are summarized in Table 2.