| Target gene | primers | Thermal profile |
| qPCR | nosZ-F [29] nosZ1622R [29] | qPCR: 94°C/2 min; 6 cycles of 94°C/30 s, 57°C/30 s (−1°C/cycle), and 72°C/45 s; 30 cycles of 94°C/30 s, 52°C/30 s, and 72°C/45 s. | DGGE | nosZ-F [29] nosZ1622-GC [30] | DGGE: 94°C/2 min; 10 cycles of 94°C/30 s, 58°C/30 s (−0.5°C/cycle), and 72°C/60 s; 30 cycles of 94°C/30 s, 53°C/30 s, and 72°C/60 s; 72°C/10 min. |
| qPCR | Cd3aF [31] R3cd [31] | q-PCR: 94°C/2 min; 6 cycles of 94°C/30 s, 57°C/30 s (−1°C/cycle), and 72°C/45 s; 30 cycles of 94°C/30 s, 52°C/30 s, and 72°C/45 s. | DGGE | Cd3aF [31] R3cd-GC [32] | DGGE: 94°C/2 min; 10 cycles of 94°C/30 s, 57°C/30 s (−0.5°C/cycle), and 72°C/45 s; 30 cycles of 94°C/30 s, 52°C/30 s, and 72°C/45 s; 72°C/10 min. |
| qPCR | F1aCu [33] R3Cu [33] | q-PCR: 95°C/3 min; 6 cycles of 95°C/30 s, 63°C/30 s (−1°C/cycle), and 72°C/30 s; 32 cycles of 95°C/30 s, 58°C/30 s, and 72°C/30 s. | DGGE | F1aCu [33] R3Cu-GC [33] | DGGE: 95°C/3 min; 32 cycles of 95°C/30 s, 58°C/30 s, and 72°C/45 s; 72°C/10 m. |
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