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Primers | Sequence (5′-3′) | Description |
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WRKY2-FP | CCCATAATCATCCTAAGCCTCTG | Forward primer for GbWRKY2 cDNA |
WRKY2-RP | ATGGGACACTGTATCTTGAAGTTGAG | Reverse primer for GbWRKY2 cDNA |
WRKY2-3 | AGAAAGTAGTGAAAGGCAACCCTAACCCAA | Forward primer for 3′-RACE, outer |
WRKY2-3N | CCATAGCACTGGCTGGTTCTCAACTTCAAG | Forward primer for 3′-RACE, nested |
WRKY2-5 | CAACTTTGATGACAGCACCGACGCC | Forward primer for 5′-RACE, outer |
WRKY2-5N | CGGTTAGGCAGAGGCTTAGGATGATTA | Forward primer for 5′-RACE, nested |
WRKY2-G1 | ATGGGAGTTGGTGCTGCT | Forward primer for GbWRKY2 gDNA |
WRKY2-G2 | TCAATGAAAAGAGGTTTCAAAGC | Reverse primer for GbWRKY2 gDNA |
WRKY2-SP1 | CCCTTCCATCATCGTCGTCACTTGC | Reverse primer for 5′-promoter, outer |
WRKY2-SP2 | ACGCCTTCCGCCCTTTCTCCTGCTT | Reverse primer for 5′-promoter, nested |
WRKY2-U | AGTGCCAGCAATCAGCGTT | Gene forward primer for qRT-PCR |
WRKY2-D | AGCCCTTGATGGGTTTCTGT | Gene reverse primer for qRT-PCR |
WRKY16-U | ATCAGTCCACAAGCAGACCTCA | Gene forward primer for qRT-PCR |
WRKY16-D | TTGTCATTAGCAACACTGCCATC | Gene reverse primer for qRT-PCR |
WRKY21-U | GAATAGTATGGGTTCCAGTAGGTCG | Gene forward primer for qRT-PCR |
WRKY21-D | AACCTTTGATAGGCTTCTGTCCA | Gene reverse primer for qRT-PCR |
GAPDH-U | GGTGCCAAAAAGGTGGTCAT | Gene forward primer for qRT-PCR |
GAPDH-D | CAACAACGAACATGGGAGCAT | Gene reverse primer for qRT-PCR |
WRKY2P-1363 | AACTGCAGACTATAGGGCACGCGTGGTC | Forward primer for promoter deletion |
WRKY2P-1018 | AACTGCAGTTCTTACGAAAGTGGTGCTGCTA | Forward primer for promoter deletion |
WRKY2P-668 | AACTGCAGACGCATGTAGCCCTAAACCAAG | Forward primer for promoter deletion |
WRKY2P-521 | AACTGCAGTTTGCGGTGCTTGAGCTAATT | Forward primer for promoter deletion |
WRKY2P-288 | AACTGCAGTGTGGTGGATGCTACACCTGAG | Forward primer for promoter deletion |
WRKY2P-137 | AACTGCAGCATAATGCCCTGTTTCCTCCAA | Forward primer for promoter deletion |
WRKY2P-48 | AACTGCAGGCAGTGAGTATCCTCGGAGTTAT | Forward primer for promoter deletion |
WRKY2-5UTR-F | AACTGCAGAACTAAGAAGAAGGTTGAACGGT | Forward primer for 5′-UTR |
WRKY2P-anti | CGGGATCCGGACAATTCAAATGTGTGCACTT | Reverse primer for promoter deletion |
WRKY2-5UTR-R | CGGGATCCTCGACGGTTAGGCAGAGGCTTA | Reverse primer for promoter deletion |
FWR2PIN | TGAAGCAGGAGAAAGGGCGGAAGGCG | Forward primer for probe |
RWR2PIN | CGCTGGTGCCCTTCCATCATCGTCG | Reverse primer for probe |
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