Research Article

Isolation, Expression, and Promoter Analysis of GbWRKY2: A Novel Transcription Factor Gene from Ginkgo biloba

Table 1

The primers used in this study.

PrimersSequence (5′-3′)Description

WRKY2-FPCCCATAATCATCCTAAGCCTCTG Forward primer for GbWRKY2 cDNA
WRKY2-RPATGGGACACTGTATCTTGAAGTTGAGReverse primer for GbWRKY2 cDNA
WRKY2-3AGAAAGTAGTGAAAGGCAACCCTAACCCAAForward primer for 3′-RACE, outer
WRKY2-3NCCATAGCACTGGCTGGTTCTCAACTTCAAGForward primer for 3′-RACE, nested
WRKY2-5CAACTTTGATGACAGCACCGACGCC Forward primer for 5′-RACE, outer
WRKY2-5NCGGTTAGGCAGAGGCTTAGGATGATTAForward primer for 5′-RACE, nested
WRKY2-G1ATGGGAGTTGGTGCTGCTForward primer for GbWRKY2 gDNA
WRKY2-G2TCAATGAAAAGAGGTTTCAAAGCReverse primer for GbWRKY2 gDNA
WRKY2-SP1CCCTTCCATCATCGTCGTCACTTGCReverse primer for 5′-promoter, outer
WRKY2-SP2ACGCCTTCCGCCCTTTCTCCTGCTTReverse primer for 5′-promoter, nested
WRKY2-UAGTGCCAGCAATCAGCGTTGene forward primer for qRT-PCR
WRKY2-DAGCCCTTGATGGGTTTCTGTGene reverse primer for qRT-PCR
WRKY16-UATCAGTCCACAAGCAGACCTCAGene forward primer for qRT-PCR
WRKY16-DTTGTCATTAGCAACACTGCCATCGene reverse primer for qRT-PCR
WRKY21-UGAATAGTATGGGTTCCAGTAGGTCGGene forward primer for qRT-PCR
WRKY21-DAACCTTTGATAGGCTTCTGTCCAGene reverse primer for qRT-PCR
GAPDH-UGGTGCCAAAAAGGTGGTCATGene forward primer for qRT-PCR
GAPDH-DCAACAACGAACATGGGAGCATGene reverse primer for qRT-PCR
WRKY2P-1363AACTGCAGACTATAGGGCACGCGTGGTCForward primer for promoter deletion
WRKY2P-1018AACTGCAGTTCTTACGAAAGTGGTGCTGCTAForward primer for promoter deletion
WRKY2P-668AACTGCAGACGCATGTAGCCCTAAACCAAGForward primer for promoter deletion
WRKY2P-521AACTGCAGTTTGCGGTGCTTGAGCTAATTForward primer for promoter deletion
WRKY2P-288AACTGCAGTGTGGTGGATGCTACACCTGAGForward primer for promoter deletion
WRKY2P-137AACTGCAGCATAATGCCCTGTTTCCTCCAAForward primer for promoter deletion
WRKY2P-48AACTGCAGGCAGTGAGTATCCTCGGAGTTATForward primer for promoter deletion
WRKY2-5UTR-FAACTGCAGAACTAAGAAGAAGGTTGAACGGTForward primer for 5′-UTR
WRKY2P-antiCGGGATCCGGACAATTCAAATGTGTGCACTTReverse primer for promoter deletion
WRKY2-5UTR-RCGGGATCCTCGACGGTTAGGCAGAGGCTTAReverse primer for promoter deletion
FWR2PINTGAAGCAGGAGAAAGGGCGGAAGGCGForward primer for probe
RWR2PINCGCTGGTGCCCTTCCATCATCGTCGReverse primer for probe