Research Article

Isolation, Expression, and Promoter Analysis of GbWRKY2: A Novel Transcription Factor Gene from Ginkgo biloba

Table 3

Putative cis-acting regulatory elements identified in the promoter of GbWRKY2 using PLACE (http://www.dna.affrc.go.jp/PLACE/) and the Signal Scan Program PlantCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/) database.

Name of cis-elementPositionSignal sequenceFunctionReferences

ABRELATERD1585ACGTGABA-responsive elements[24]

ABRERATCAL 10MACGYGBCa2+-responsive and ABA upregulated genes[25]

ARR1AT 629, 634, 564, 90, 751, 883NGATTARR1 binding element involved in cytokinin signaling[26]

ACGTATERD1262, 585ACGTInvolved in upregulation by dehydration stress and dark-induced senescence[27]

BIHD1OS595, 772, 863, 1307TGTCABELL recognition site involved in disease resistance responses[28]

CAATBOX1232, 787, 824CAATCommon cis-acting element in promoter and enhancer regions[29]

CRTDREHVCBF218GTCGACLow-temperature responsive [30]

DOFCOREZM 354, 621, 687, 1258, 1301AAAG Dof1 and Dof2 binding element involved in carbon metabolism[31]

DPBFCOREDCDC31089ACACNNGDPBF-1 binding core sequence involved in ABA signaling[32]

EBOXBNNAPA 86, 232, 318, 714, 729, 775, 1090, 1246, 1280, 1350CANNTGCis-element binding BHLH factor is dispensable for light responsiveness[33]

GATABOX124, 222, 290, 640, 681, 1253, 1276GATACommon cis-acting element in promoter [34]

GT1CONSENSUS 475, 803, 928GRWAAWConsensus GT-1 binding site in many light-regulated genes and influence the level of SA-inducible gene expression[35]

IBOX124GATAAGConserved sequence upstream of light-regulated genes[36]

MYB2CONSENSUSAT 318, 1246, 1280, 591YAACKGMYB recognition site involved in dehydration responsiveness[37]

MYCCONSENSUSAT86, 232, 318, 714, 729, 775, 1090, 1246, 1280CANNTGMYC recognition site involved in dehydration responsiveness and cold responsiveness[38]

PYRIMIDINE-box214, 840, 869, 890CCTTTTGibberellin-response cis-element[39]

ROOTMOTIFTAPOX1178, 223, 546, 641, 722ATATTRoot specific expression[40]

TATA box 94TTATTTCommon cis-acting element in promoter and enhancer regions[41]

WBOXATNPR1887TTGACYWRKY binding site, involved in many physiological processes[10]