The SsDREB Transcription Factor from the Succulent Halophyte Suaeda salsa Enhances Abiotic Stress Tolerance in Transgenic Tobacco

Figure 2

Analysis of SsDREB binding to the DRE element in the yeast one-hybrid system. (a) Construction of the YepGAP-SsDREB plasmid. The entire SsDREB coding region was fused to the activation domain of GAL4. Recombinant YepGAP-SsDREB or plasmid was transformed into yeast cells that are harboring two reporter genes under the control of either wild-type or mutant DRE motifs. PGAP and TADH1 indicate the promoter and terminator of ADH1 gene, respectively. (b) Transformed yeast cells were examined for growth on selective medium (SD−His+10 mM 3-AT) at 30°C (left). Left panel shows the position of each transformed yeast cell. The empty YepGAP (PAD) was used as a control. wDRE and wPAD indicate yeast cells harboring DREB proteins and DRE-controlled reporter genes, while mDRE and mPAD indicate yeast cells harboring DREB proteins and mDRE-controlled reporter genes.

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